Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Aug 7;7:7450. doi: 10.1038/s41598-017-07077-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Sterol microarrays suggest that the transmembrane CRAC domain from SIDT1 binds several sterols. (A) Cartoon illustrating the putative secondary structure of SIDT1. The nine putative transmembrane domains are shown. In red are mark the amino acids forming the two CRAC domains and in blue a putative lysosome localization signal (not explored in the present study). (B) TIRFM-based microarray studies illustrating the spots containing the following sterols tested: cholesterol, cholic acid, dehydrocholic acid, β-estradiol and testosterone. As positive control, some spots in the microarray were printed with an anti-GFP antibody (lower panels). Each microarray contained 2 lines of 17 spots each. The fluorescence illustrated represents the binding of the fusion protein formed by the CRAC domain attached to GFP. For CRAC domain sequences, please refer to Material and Methods. Notice that the extracellular CRAC domain did not bind any of the sterols tested while the transmembrane CRAC domain binds to cholesterol and only weakly to cholic acid. Both fusion proteins bind strongly to the anti-GFP antibody.