Table 3.
Metabolite content changes in roots (A) or leaves (B) after seed inoculation by A. lipoferum CRT1.
| Organ | Substance name | FC | L | C | Corg |
|---|---|---|---|---|---|
| Root | Cadaverine | 270.6 ± 1.6* | −20.2 ± 11.6 | 7.3 ± 6.6 | −1.7 ± 9.3 |
| Cellobiose# | 127.7 ± 50.1 | −63 ± 15.1′ | 35.8 ± 107.7 | 9.5 ± 47.9 | |
| 4-hydroxy- cis-cinnamic acid | 145.6 ± 30.0 | −52.5 ± 8.1′ | NA | −49.31 ± 0.3 | |
| Dihydrocoumarin | 39.2 ± 2.7* | −54.9 ± 20.1 | NA | NA | |
| Galactinol# | 129.2 ± 26.7 | −50.2 ± 26.9 | −60.1 ± 3.5* | 27 ± 1.8 | |
| Glutamine | 25.3 ± 1.9* | 3.5 ± 6.9 | 21.1 ± 5.8 | −19.5 ± 2.8 | |
| Isomaltose# | 265.8 ± 6.1* | −76.6 ± 6.0 | −22.5 ± 0.7 | 90 ± 8.1 | |
| Maltitol# | 81 ± 78.1 | −35.3 ± 7.8 | −65.8 ± 1.1* | 291.6 ± 48.4 | |
| Maltose# | 176 ± 24.7′ | −21.5 ± 24.0 | −21.5 ± 1.9 | 510 ± 69.3 | |
| Phosphoric acid | 56.6 ± 17.3 | −9.9 ± 1.7 | −0.7 ± 1.1 | 80.9 ± 7.2′ | |
| Ribose-5-phosphate# | 134.6 ± 25.1 | −16 ± 7.5 | −58.4 ± 9.7′ | 26.8 ± 23.4 | |
| Sophorose# | 198.4 ± 19.8′ | 14.7 ± 9.8 | −32.7 ± 10.4 | 222 ± 31.2 | |
| A264005 | 164 ± 78.9 | −55.2 ± 13.1′ | −10.4 ± 51.8 | 46.4 ± 20.8 | |
| Leaf | Beta- alanine | 118.0 ± 2.6* | 11.6 ± 2.5 | 91.9 ± 104.0 | −73.1 ± 59.1 |
| Cinnamic acid, trans- | 111.2 ± 4.5′ | −76.4 ± 4.2′ | 77.1 ± 7.0 | −61.6 ± 3.4′ | |
| Ferulic acid, trans- | NA | −77.5 ± 0.3 | 100.8 ± 49.8 | −66.21 ± 0.2* | |
| Fructose# | −6.7 ± 52.5 | 88.1 ± 15.9 | −32.2 ± 74.5 | −61.6 ± 13.8′ | |
| Galactinol# | 462.7 ± 77.1 | NA | 117.3 ± 49.0 | −69.2 ± 12.9′ | |
| Galactose# | 36.9 ± 35.5 | −51.0 ± 10.7 | 34.7 ± 24.7 | −62.9 ± 7.2* | |
| Glucose# | 6.9 ± 73.1 | 136.3 ± 46.8 | −28.8 ± 33.6 | −27.1 ± 1.4* | |
| Glucose-6-phosphate# | 59.0 ± 34.6 | −22.2 ± 0.8 | 23.5 ± 17.2 | −52.7 ± 1.4′ | |
| Glucuronic acid# | −266.8 ± 43.8 | −47.5 ± 0.1 | −3.0 ± 1.9 | −71.3 ± 7.2* | |
| Glutamine | 256.6 ± 1.1* | −61.4 ± 2.1′ | 85.0 ± 3.9 | −64.5 ± 31.1 | |
| Gulonic acid# | −3.9 ± 0.7 | −78.6 ± 16.3 | 233.4 ± 0.9′ | −8.9 ± 3.4 | |
| Gulonic acid, 2-oxo-# | 23.0 ± 63.5 | 89.4 ± 6.1* | −24.8 ± 94.7 | −26.9 ± 29.1 | |
| Isobutanoic acid, 3-amino- | 82.1 ± 0.2′ | −57.4 ± 5.5′ | 77.1 ± 28.9 | −77.1 ± 3.6′ | |
| Maltose# | 29.12 ± 1.3 | −11.6 ± 1.3 | −29.0 ± 8.2 | −24 ± 0.1* | |
| Mannosamine, N-acetyl-# | 531.7 ± 6.9* | −62.6 ± 9.6 | 145.5 ± 16.5 | −60.7 ± 0.6′ | |
| Mannose# | 62.4 ± 269.8 | −56.5 ± 82.9 | 33.0 ± 212.5 | −68.3 ± 7.2* | |
| Quinic acid | 61.9 ± 19.7 | −58.8 ± 8.5 | 37.9 ± 15.4 | −65.5 ± 3.9* | |
| Rhamnose# | −329.7 ± 52.0 | −62.1 ± 75.3 | 82.6 ± 220.2 | −65.8 ± 19.5′ | |
| Ribose# | 64.7 ± 69.8 | −62.9 ± 55.0 | −2.4 ± 1.2 | 50.7 ± 1.4* | |
| Tagatose# | 27.1 ± 6.6 | 11.4 ± 1.3 | −8.8 ± 22.4 | −66.8 ± 2.5* | |
| A231002 | 27.1 ± 128.5 | 82.9 ± 11.8′ | −1.8 ± 221.9 | 14.0 ± 84.0 | |
| A257011 | 62.6 ± 37.8 | −54.1 ± 52.5 | 203.5 ± 55.0 | −76.8 ± 3.6′ | |
| A115002 | 268.3 ± 2.3* | −89.3 ± 22.7 | 126.6 ± 6.1 | −40.5 ± 4.9 | |
| A214003 | 47.6 ± 18.8 | −49.7 ± 17.4 | 9.4 ± 1.3 | −54.0 ± 1.1* | |
| A250008 | 731.5 ± 21.7 | −63.4 ± 31.3 | 33 ± 18.4 | −71.7 ± 9.5′ | |
| A255001 | 226.7 ± 55.6 | −78.3 ± 8.3* | 19.8 ± 0.3 | −46.1 ± 1.4′ | |
| A268008 | 105.2 ± 1.5* | −73.4 ± 53.5 | 111.4 ± 31.5 | −84.1 ± 2.1* | |
| A311002 | 76.1 ± 7.6′ | −65.4 ± 33.4 | 18.9 ± 59.7 | −77.0 ± 58.1 |
Ratios of mean content values of inoculated over mock-inoculated plantlets are listed and expressed as percentage values +/− percentage relative standard error. Wilcoxon non-parametric tests were conducted for each metabolite to compare inoculated and non-inoculated plants mean values for each field (FC, L, C and Corg). Only substances displaying significant content changes (*p < 0.05; ′p < 0.1) are listed. NA indicates that the metabolite was detected in less than 2 of technical replicate samples. Metabolites were identified by comparison with a standard and are listed in alphabetical order. Unidentified metabolites are named according to their code in the Golm Metabolome Database (http://gmd.mpimpgolm.mpg.de/). Carbohydrates and carbohydrate-related metabolites are marked with the pound sign (#).