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. 2017 Aug 7;7:7411. doi: 10.1038/s41598-017-07606-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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AC deletion stops G1-S cell cycle progression. (A) Cell cycle progression was assessed using propidium iodide and flow cytometry analysis. Dot plots show different distribution between G1, S and G2 phases in control (scramble-treated, top) versus ASAH1-null cells (bottom). (B) Statistical analysis of the cell cycle distribution was performed using the Student’s t test; *p < 0.05, ** p < 0,01,***p < 0.001,. Experiments were performed 17 days after CRISPR/Cas9 ablation.