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. 2017 Aug 7;7:7442. doi: 10.1038/s41598-017-06637-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Functional cytoplasmic domain of CD146 in VEGF-C induced cell activation. (a) Diagrammatic representation of C-terminal truncations of CD146 at the intracellular domain. (b) Phosphorylation and expression of ERK1/2 and p38, elicited from VEGF-C156S, were analyzed by WB. HEK293 cells were transfected with plasmids encoding truncated versions of CD146 as shown in (a). (c–g) HDLECs transfected with plasmids encoding CD146-WT and CD146-∆599-646 were subjected to signaling activation assay (c), spheroid sprouting assay (d), proliferation assay (e), tube formation assay (f) and transwell migration assay (g). VEGF-C was applied at the indicated concentration. Data were expressed as means ± SEM from 3 independent experiments (n = 12 in each group of c–g,). Significant difference was determined by Student’s t-test (*P < 0.05; **P < 0.01; ***P < 0.001; ns, no significant difference). Full-length blots are presented in Supplementary Fig. S4.