Table 8.
PCR primers used in this study.
| Target gene (Primer specificity) | Primer sequence (5’ → 3’ direction)* | Amplicon size (bp) | Annealing temperature (°C) | Reference |
|---|---|---|---|---|
| mecA | Forward: GAAGATGGCTATCGTGTCACA Reverse: GGAACTTGTTGAGCAGAGGTT | 307 | 52 | Current study |
| mecC | Forward: GGGTTCAGCCAGATTCATTTGT Reverse: GTACTGTTGCTTCGTTCAATGG | 138 | 52 | Current study |
| spa | 1113_ Forward: TAAAGACGATCCTTCGGTGAGC 1514_ Reverse: CAGCAGTAGTGCCGTTTGCTT | Variable (180–670) | 52 | 91 |
| PVL | Forward: GCTGGACAAAACTTCTTGGAATAT Reverse: GATAGGACACCAATAAATTCTGGATTG | 83 | 50 | 94 |
| VanA | Forward: GGGAAAACGACAATTGC Reverse: GTACAATGCGGCCGTTA | 732 | 50 | 95 |
| SCCmec I | CIF2 F2: TTCGAGTTGCTGATGAAGAAGG CIF2 R2: ATTTACCACAAGGACTACCAGC | 495 | 47 | 96 |
| SCCmec II | KDP F1: AATCATCTGCCATTGGTGATGC KDP R1: CGAATGAAGTGAAAGAAAGTGG | 284 | 47 | 96 |
| SCCmec III | RIF5 F10: TTCTTAAGTACACGCTGAATCG RIF5 R13: GTCACAGTAATTCCATCAATGC | 414 | 47 | 96 |
| SCCmec I, II, IV | DCS F2: CATCCTATGATAGCTTGGTC DCS R1: CTAAATCATAGCCATGACCG | 342 | 47 | 96 |
| SCCmec V | ccrC F2: GTACTCGTTACAATGTTTGG ccrC R2: ATAATGGCTTCATGCTTACC | 449 | 47 | 89 |
| tst | Forward: ACCCCTGTTCCCTTATCATC Reverse: TTTTCAGTATTTGTAACGCC | 326 | 47 | 97 |
*F: forward primer; R: reverse primer.