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. 2017 Mar 14;22(3):462. doi: 10.3390/molecules22030462

Figure 3.

Figure 3

FKA increases MPM-2 phosphorylation and Cdc kinase activity via inhibition of Cdc2 and Cdc25 phosphorylation and downregulation of Myt1 and Wee1 expression. (A) Cdc2 associated histone H1 kinase activity was decreased dose-dependently by FKA treatment for 24 h in SKBR3 cells; (B–D) SKBR3 cells were treated with indicated concentrations of FKA for 24 h. Phosphorylation levels of Cdc2, MPM-2, and Cdc25C as well as protein levels of cyclin B1, Myt1, and Wee1 were detected by Western blotting analysis. β-actin was detected as a loading control. A representative blot was shown from three independent experiments.