Figure 5.

FKA plus Herceptin results in enhanced growth inhibitory effect on HER2-overexpressing breast cancer cell lines via downregulation of Myt1, Wee1, surviving, and XIAP. (A) MCF7/HER2 and SKBR3 cells (5 × 10⁴) were plated in 24-well culture plates. After 24 h, cells were treated with 0.05% DMSO, Herceptin alone, FKA alone, and FKA plus Herceptin, at the indicated concentrations. After 72 h of treatment, cell densities were measured by MTT assay. Points represent the mean of four independent plates; bars, SE. Each sample was counted in duplicate. “**” denotes “p < 0.01” for Hercepin or FKA alone vs the combination; (B) SKBR3 cells were treated with indicated concentrations of Herceptin, FKA, or their combination for 24 h. Western blotting analysis of Myt1, Wee1, survivin, and XIAP is shown by a representative blot from three experiments. β-actin was detected as a loading control.