Fig. 3. SIRT1-independent changes in ROS levels and Δψm by NAM treatment.
Fibroblasts were treated with either resveratrol at 5 or 10 μM (A), or SRT1720 at 0.08 or 0.16 μM (B), for 1 or 2 days, and were subjected to flow cytometry after staining with NAO, DHE, or JC-1. Cells treated with 5 mM NAM alone were analyzed in parallel. (C and D) Fibroblasts were transfected either with random RNA or with siRNA to SIRT1, for 12 h; fibroblasts were then further incubated in the absence (light bar) or the presence (dark bar) of 5 mM NAM. After 1 or 2 d, cells were collected, treated with DHE (for C) or JC-1 (for D), and subjected to flow cytometry for quantitative comparison of the levels of superoxide (C) and Δψm(D). ). *P < 0.05, **P < 0.01 (compared to Day 0 control, one-way ANOVA.