Fig. 1.

EE treatment does not affect mammary gland morphology at lactational day 21. A) Representative whole mount mammary glands collected from females exposed to vehicle, 0.01 or 1 μg EE/kg/day from pregnancy day 9 through lactational day 20. Mammary glands were collected on lactational day 21, prior to weaning, fixed and stained with carmine alum. Zeiss ZEN software was used to quantify intensity of gland (a measure of epithelial density) at four discrete locations. Scale bars represent 2 mm. B) Quantification of data collected from whole mounts. Intensity has arbitrary units. Statistical significance was evaluated using 1-way ANOVA and Bonferroni posthoc tests, and no significant differences between groups were revealed.