Subject area | Biology |
More specific subject area | Endocrinology, reproductive science, endocrine disruptors |
Type of data | Figures, graphs Whole mount mammary glands Histological stain: Hematoxylin & Eosin Immunohistochemistry: Ki67 (marker of proliferation) and Estrogen Receptor α qRT-PCR: Esr1 |
How data was acquired | Zeiss AxioImager dissection microscope (whole mount glands) Zeiss Axio Oberserver.Z1 inverted microscope (histology and immunohistochemistry) Zeiss high resolution color camera |
Data format | Primary data, quantified and analyzed graphs |
Experimental factors |
Exposure of female CD-1 mice to 0.01 or 1 μg ethinyl estradiol/kg/day from pregnancy day 9 through lactational day 20; oral route of exposure Mammary glands collected on lactational day 21 Whole mount mammary glands stained with carmine alum; mammary glands fixed in neutral buffered formalin, paraffin embedded and sectioned |
Experimental features | Assessment of mammary gland morphology; quantification of epithelial cell proliferation; expression of estrogen receptor α in females exposed to vehicle (control) compared to females exposed to one of two doses of ethinyl estradiol |
Data source location | Amherst, MA, USA |
Data accessibility | Data are present in this article |