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. 2017 Jul 27;14:337–343. doi: 10.1016/j.dib.2017.07.062
Subject area Biology
More specific subject area Endocrinology, reproductive science, endocrine disruptors
Type of data Figures, graphs
Whole mount mammary glands
Histological stain: Hematoxylin & Eosin
Immunohistochemistry: Ki67 (marker of proliferation) and Estrogen Receptor α
qRT-PCR: Esr1
How data was acquired Zeiss AxioImager dissection microscope (whole mount glands)
Zeiss Axio Oberserver.Z1 inverted microscope (histology and immunohistochemistry)
Zeiss high resolution color camera
Data format Primary data, quantified and analyzed graphs
Experimental factors Exposure of female CD-1 mice to 0.01 or 1 μg ethinyl estradiol/kg/day from pregnancy day 9 through lactational day 20; oral route of exposure
Mammary glands collected on lactational day 21
Whole mount mammary glands stained with carmine alum; mammary glands fixed in neutral buffered formalin, paraffin embedded and sectioned
Experimental features Assessment of mammary gland morphology; quantification of epithelial cell proliferation; expression of estrogen receptor α in females exposed to vehicle (control) compared to females exposed to one of two doses of ethinyl estradiol
Data source location Amherst, MA, USA
Data accessibility Data are present in this article