Fig. 5.

Neurons recorded in whole cell configuration were of good quality. A: example of spontaneous activity from a neuron recorded 3.2 mm below the pial surface with detail of spontaneous burst (arrow indicates burst shown in detail on right). B: example whole cell traces recorded in the thalamus for 3 different neurons. Note the sag in membrane potential following hyperpolarizing current injection (representing activity of I_h, as described in Leist et al. 2016) and afterhyperpolarization rebound bursting in each trace, indicative of thalamic neurons. Current injections lasted 0.5 s and ranged from ±50 pA (1st recording) to ±100 pA (2nd and 3rd recordings). C: after hyperpolarizing current injection, rebound bursts exhibited afterdepolarization (see arrow, consistent with ventrobasal thalamic nucleus cells; Wang et al. 2010b). Current injection was −150 pA.