Figure 4. AP7Glu induces cell death of HCT116 cells. A) Representative composite images showing morphological changes of HCT116 cells detected with DAPI staining. Cells were treated with IC50 concentrations of apigenin or AP7Glu for 48 h, and imaged by fluorescence microscope. Apoptotic bodies formation are marked by yellow arrows. B) Flow cytometry analysis of Annexin-FITC staining and propidium iodide accumulation after treatment of HCT116 cells with apigenin or AP7Glu. Cells were treated either with DMSO or corresponding IC50 concetrations (treatment) for 48 h. One representative analysis was presented in upper panel. Results of quantitative analyses of PI and Annexin positive cells were presented as the means ± SEM of at least three independent experiments. P values were calculated using Student's t-test, *p ≤ 0.05. Q1: PI+cells; Q2: PI+/Annexin+cells; Q3: PI-/Annexin-cells; Q4: Annexin+cells.
