Fig. 6.

Amplification of RPN13 does not alter expression of protein. a Amplification of ADRM1 was assayed by Taqman Gene Assay and analyzed by CopyCaller™ Software for 7 ovarian cancer and 1 immortalized fallopian tube epithelial (FT2021) cell lines measured. Similarly, purified mRNA was measured by qRT-PCR and found to have no correlation with amplification status. b As assayed by Western blot, RPN13 protein levels vary little despite ADRM1 gene amplification status. Ratio to loading control, ß-actin, was calculated by comparing pixel density as measured by ImageJ. C) XTT cell viability assay of various HGSC cell lines with known RPN13 CNV. All cell lines were shown to be sensitive to RPN13 inhibition by RA190 regardless of amplification status