Fig. 7.

SIAH1, SIAH2 and SIAH3 mRNA expression in human cancer cell lines. Semi-quantitative reverse transcription – polymerase chain reaction (RT-PCR) analysis of siah1, siah2 and siah3 mRNA transcript expression in human cancer cell lines is shown. The relative expression levels of siah1, siah2 and siah2 mRNA transcripts in 13 epithelial cancer cells including pancreatic cancer cells (MiaPaCa, AsPC-1, PANC-1 and CFPAC), prostate tumor cell lines (LNCaP, LAPC-4. PC-3-N (normal), PC-3-ML (metastatic)), breast tumor cell lines (MCF-7 and MDA-MB-231), non-small cell lung cancer cell line (A549) and cervical cancer cell line (HeLa) were estimated semi-quantitatively for serial dilutions of the complementary DNA templates. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA transcript was used as an internal control. The RT-PCR mixture without cDNA template was used as a negative control