Table 1. Growth of hES colonies under conditions of increasing hypoxia.
| O2 concentration, % | Colonies, n | Colonies per well, n | Colony surface area, μm2 (n) | DNA per well, μg | DNA per colony, ng | Est. cells per well, n |
|---|---|---|---|---|---|---|
| 21 | 238 | 79.3 ± 15.5 | 72,189 ± 4,343 (35) | 14.2 | 179 | 2.37 × 106 |
| 3 | 260 | 86.7 ± 5.9 | 71,932 ± 3,669 (35) | 14.9 | 172 | 2.48 × 106 |
| 1 | 260 | 86.7 ± 6.4 | 62,833 ± 5,656 (36) | 9.45 | 109 | 1.57 × 106 |
A comparison of hES cell colony growth under normoxic and increasingly severe hypoxic conditions. H1 cells (passage 36) were subcultured into nine wells in three independent six-well plates (three wells per plate) and cultured under 21%, 3%, or 1% O2, respectively. Colony sizes were measured on day 15. DNA from colonies was pooled prior to assay and corrected for the amount of MEF cell DNA (0.735 μg per well) present in equivalent wells without ES cells. The number of colonies (P = 0.85) and colony surface area (P = 0.27) did not differ between O2 concentrations. Under colony surface area, the values in parentheses are the numbers of colonies examined. Est., Estimated.