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. 2017 Aug 8;22:14. doi: 10.1186/s11658-017-0044-2

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Overexpression of WNT1 inhibited the osteogenic differentiation capacity of SCAPs. a Western blot results confirmed the overexpression of WNT1 via retrovirus infection of SCAPs. Beta-actin was used as an internal control. b ALP activity. c Alizarin Red staining. d Quantitative analysis of calcium concentration. Student’s t test was performed to determine statistical significance. All error bars represent SD (n = 3). **p ≤ 0.01. (sigma unit: unit of measurement for ALP activity)