Figure 2A, 2B.

Resistance to TET was higher, but proliferation was slower, in TET-resistant (TET-R) Jurkat cells. TET-R Jurkat cells resistant to 5 μmol/L TET were established as a model for investigating drug resistance. TET-R Jurkat cells (3×10⁵ cell/mL) incubated in a 96-well plate were subjected to higher concentrations of TET, as indicated, for 24 h. (A) Cell viability was determined by performing MTT assays, and the IC₅₀ against TET in TET-R Jurkat cells was calculated. (B) Cell proliferation was measured by trypan blue exclusion, and the cell doubling time of TET-R Jurkat cells was calculated. TET-S and TET-R Jurkat cells (3×10⁵ cell/mL) were incubated in 6-well plates overnight and treated with 5 μmol/L TET for 24 h, after which they were sorted and fixed with alcohol.