Figure 3.

Suppression of AP-1 activity by TET in Jurkat cells. Electrophoretic mobility shift assay of DNA-binding activity of AP-1 (A) and NF-κB (B) in nuclear extracts from Jurkat cells treated with 10 μmol/L TET for 2, 6, 12, 18 and 24 h is shown. Some reactions included an unlabelled competitor (wild-type oligonucleotide probe) and an unlabelled mutant (non-specific) probe. Quantitative densitometry data are expressed as the mean±SD (n=2 in CEM groups, n=3 in Jurkat groups). ^*P<0.05 vs control.