Figure 6.

Effects of specific MAPK inhibitors on AP-1 activity and viability of TET-R Jurkat and CEM cells treated with TET. CEM cells (3×10⁵ cells/mL) were incubated in 96-well plates or 10-cm cell culture dishes and treated with or without 20 μmol/L SP600125 (a specific JNK inhibitor) for 24 h in the presence or absence of 10 μmol/L TET in the medium. The DNA-binding activity of AP-1 was detected with an EMSA. Cell viability and TET toxicity were analysed using MTT and LDH assays, respectively.