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. 2017 Jun 12;38(8):1161–1170. doi: 10.1038/aps.2017.62

Figure 3.

Figure 3

miR-511 inhibits the expression of BTG1 by directly targeting the 3′UTR of its mRNA. (A) A schematic representation of the predicted binding site of miR-511 in the 3′UTR of BTG1 mRNA. (B) The mutant seed region was generated at the 3′UTR of BTG1 mRNA as indicated. A BTG1 3′UTR fragment containing the wild-type or mutant miR-511-binding sequence was cloned into the downstream region of the pGL3-control luciferase reporter gene vector. (C and D) The effect of miR-511 on the pGL3-BTG1-wt and pGL3-BTG1-mut reporters in HepG2 and H7402 cells was measured using luciferase reporter gene assays. (E and F) The effect of anti-miR-511 on the pGL3-BTG1-wt and pGL3-BTG1-mut reporters in HepG2 and H7402 cells was examined using luciferase reporter gene assays. Data are presented as the mean±SD of three independent experiments. *P<0.05, **P<0.01, NS, no significance; Student's t test.