Figure 5.

Osthole attenuated the phosphorylation of p38 in TNBS-induced colitis. Mice received osthole intraperitoneally at 100 mg/kg once daily starting at three days before being exposed to TNBS treatments and until the end of the experiments. Colitis was induced by infusing a 100 μL enema of TNBS (2.8 mg) in 50% ethanol into the colonic lumen. Western blot analysis of the colonic mucosa homogenates was performed 2 d after the initial rectal TNBS administration.