Table 2. Primers used to detect gene expression at mRNA level.
Gene name | Primer sequences (5′-3′) |
---|---|
IL-6 | F: CTGCAAGAGACTTCCATCCAG |
R: AGTGGTATAGACAGGTCTGTTGG | |
IL-1β | F: TTCAGGCAGGCAGTATCACTC |
R: GAAGGTCCACGGGAAAGACAC | |
MCP-1 | F: ACTGAAGCCAGCTCTCTCTTCCTC |
R: TTCCTTCTTGGGGTCAGCACAGAC | |
CXCL10 | F: CCAAGTGCTGCCGTCATTTTC |
R: GGCTCGCAGGGATGATTTCAA | |
COX2 | F: TTCCAATCCATGTCAAAACCGT |
R: AGTCCGGGTACAGTCACACTT | |
TNFα | F: CCAGGCGGTGCCTATGTCTC |
R: CAGCCACTCCAGCTGCTCCT | |
Claudin1 | F: TGCCCCAGTGGAAGATTTACT |
R: CTTTGCGAAACGCAGGACAT | |
ZO1 | F: GCCGCTAAGAGCACAGCAA |
R: TCCCCACTCTGAAAATGAGGA | |
GAPDH | F: AGGTCGGTGTGAACGGATTTG |
R: GGGGTCGTTGATGGCAACA |