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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Exp Neurol. 2016 Oct 18;288:122–133. doi: 10.1016/j.expneurol.2016.10.007

Figure 1. Grading and color coding.

Figure 1

C-Fos staining was graded on two independent scales, 0.5 to 2.5 for the density (number of stained cells/unit area) and 0.5 to 2.5 for intensity (darkness of staining/cell) in all analyzed brain regions. Examples of density scoring are shown in red font and of intensity scoring in black font. The numbers for density and intensity were added to provide single scores, which were plotted in Table 2.