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. 2016 Dec 23;1:16030. doi: 10.1038/sigtrans.2016.30

Figure 2.

Figure 2

PD-L1lo B16 and ID8agg TICs are functionally defective. Representative photomicrographs of tumorospheres after 14 days in culture of B16 (a) or ID8agg (b) control (ctrl) and PD-L1lo clones. Bars equal 100 μm. Summary data below. At least six fields assessed per clone. P-values, unpaired t-test. (c) Survival of WT mice injected with 5000 indicated, sorted TICs (n=5–6 per group). P-value, log-rank test. (d) Tumor growth in NSG mice injected with indicated, sorted 104 B16 TICs (n=5 per group). P-value, two-way analysis of variance. (e) Survival of mice in d. (f) % Survival in NSG mice injected with TICs sorted from tumors in d. P-value, log-rank test for trend. (g) Survival of WT mice injected intraperitoneal with 4×106 total indicated ID8agg cells. (h) Survival of WT mice injected with 0.15×106 ID8agg ctrl or PD-L1lo clone 3 TIC. P-value, log-rank test. In e–h, boxed numbers indicate median survival (days).