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. 2017 Jul 18;114(31):E6332–E6341. doi: 10.1073/pnas.1619932114

Fig. 4.

Fig. 4.

Interaction of wild-type NUPR1 and C-RING1B mapped by HSQC spectra of C-RING1B. (A) Overlay of C-RING1B selected spectra in the presence of different amounts of NUPR1: 0 (black), 20 (red), and 61 (blue) μM. (B) Fitting of the absolute intensity (corrected by the value of the receiver gain of the spectrometer in each titrating point) of the cross-peak of C-RING1B, appearing at 9.40 ppm to Eq. 1; the Kd was 31 ± 16 μM. a.u., arbitrary units.