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In situ PLA of C-RING1B and wild-type NUPR1. (A) Mouse anti-HA and rabbit anti-human wild-type NUPR1 were used to reveal the interaction between the proteins in HeLa cells. (B) PLA was carried out as described (34); cells were treated beforehand with TFP 50 μM for 24 h. ImageJ was used to count the number of red dots. Data are the means of 10 fields each containing not fewer than 150 nuclei counted (*P ≤ 0.001). (Magnification: A, 60×; B, 20×.)
