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Far-UV CD characterization of the binding between C-RING1B and NUPR1 mutants by using steady-state spectra. (A) Far-UV CD spectra of the complex (black line) and that obtained by the addition of the spectra of each protein (red line) in the mutant Thr68Gln. (B) Far-UV CD spectra of the complex (black line) and that obtained by the addition of the spectra of each protein (red line) in the mutant Ala33Gln/Thr68Gln. mdeg, millidegrees.
