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Specific interaction between C-RING1B and wild-type NUPR1. In situ PLA in CRISPR/Cas9n control cells (with wild-type NUPR1) and CRISPR/Cas9n NUPR1 knockout cells. Mouse anti-HA and rabbit anti-NUPR1 were used to detect the protein interaction by PLA. DAPI staining was used to detect nuclei and combined with the PLA fluorescence in the merged panel. (Magnification: 40×.)
