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Immunofluorescence in HeLa cells transfected with wild-type and NUPR1 mutants. Mouse anti-FLAG primary antibody and Alexa 488-labeled goat anti-mouse secondary antibody were used to reveal the localization of the protein. DAPI staining was used to detect nuclei and it was combined with the Alexa 488 fluorescence in the merged panel. (Magnification: 20×.)
