Fig. S2.
Opioid antagonism reverses the antihyperalgesic effect induced by morphine but not the effect induced by ibuprofen. Inhibitory effects of ibuprofen (IB) and morphine (Mor) on mechanical (A) and thermal (B) inflammatory hyperalgesia. Effects of sigma-1 agonist PRE-084 (PRE) or opioid antagonists naloxone (Nx) or naloxone methiodide (Nx-M) with IB or Mor on mechanical (C) or thermal (D) hyperalgesia. Mice were injected i.pl. with carrageenan (inflamed) or saline (noninflamed) and s.c. with the drugs or solvent controls. Bars show means ± SEM from 8 to 10 animals. *P < 0.05, **P < 0.01, mice without vs. mice with inflammation; #P < 0.05, ##P < 0.01, mice with inflammation treated with IB or Mor vs. mice with inflammation treated with solvent controls; ††P < 0.01, mice treated with Nx or Nx-M vs. mice treated with solvent controls, among those mice with inflammation and treated with Mor; one-way ANOVA followed by Bonferroni test.