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. 2017 Jul 17;114(31):8396–8401. doi: 10.1073/pnas.1620068114

Fig. S3.

Fig. S3.

The in vivo treatment with anti-Ly6G decreases neutrophil load in the paw during carrageenan-induced acute inflammation without altering the number of macrophages/monocytes. (A) Representative FACS diagram of CD45+ cell populations showing that neutrophils CD11b+Ly6G+ are the most abundant leukocytes in the inflamed paw 3 h after carrageenan injection, whereas macrophages/monocytes (CD11b+Ly6G–) constitute a minor population. (B) Effects of in vivo treatment with anti-Ly6G on neutrophils and macrophages/monocytes (macs/mono) in the inflamed paw. Mice were treated intraperitoneally with anti-Ly6G antibody or its solvent control and injected i.pl. with carrageenan 3 h before obtaining the samples. Graph shows means ± SEM from n = 4 determinations. **P < 0.01, number of neutrophils in mice treated with anti-Ly6G vs. solvent control. There were no statistical differences (N.S.) between the number of macrophages from mice treated with anti-Ly6G vs. solvent control; one-way ANOVA followed by Bonferroni test.