Table S2.
Primers used in this study
| Primer name | Sequence (5′ to 3′) |
| For protein expression constructs (pET-DUET vector) | |
| XylFII-F-BglII | CCCAGATCTAATTTATTAAGAGACAAGAAAG |
| XylFII-R-XhoI | AAACTCGAGTTAGTTTTCAACTTTATCATC |
| LytS-F-BamHI | AAAGGATCCATGCTTAACAATATGCTCATTAC |
| LytS-R-SalI | AAAGTCGACTTAGCTGCTATTTCTATTTAGTTC |
| For protein expression constructs (pXY1 vector) | |
| XylFII-Full-F-BamHI | AGGAGGTTAGTTAGAGGATCCATGAAATTCTTAAAGAGCGTTT |
| XylFII-Full-R-BamHI | CCAGTGAATTCCCGGGGATCCTTAGTTTTCAACTTTATCATCTCC |
| XylFII-F1 | AATTTATTAAGAGACAAGAAAGTAG |
| XylFII-R1 | CTACTTTCTTGTCTCTTAATAAATT |
| For protein expression constructs (pET-28a vector) | |
| LytS-F-BamHI | AAAGGATCCATGCTTAACAATATGCTCATTAC |
| LytS-R-SalI | AAAGTCGACTTAGCTGCTATTTCTATTTAGTTC |
| For protein expression constructs (pGEX4T-1 vector) | |
| XylFII-F-BglII | CCCAGATCTAATTTATTAAGAGACAAGAAAG |
| XylFII-R-XhoI | AAACTCGAGTTAGTTTTCAACTTTATCATC |
| For site-directed mutagenesis experiments | |
| tXylFII-H22A-F | GTGCTGATTTCTGCTATAAAGACTAATC |
| XylFII-H22A-R | GATTAGTCTTTATAGCAGAAATCAGCAC |
| XylFII-Y28A-F | AAAGACTAATCCAGCTTGGCTTGATATAAA |
| XylFII-Y28A-R | TTTATATCAAGCCAAGCTGGATTAGTCTTT |
| XylFII-W29A-F | CTAATCCATATGCGCTTGATATAAAAG |
| XylFII-W29A-R | CTTTTATATCAAGCGCATATGGATTAG |
| XylFII-D103A-F | GTAGTTACAATAGCTTCAGATGAAGAAG |
| XylFII-D103A-R | CTTCTTCATCTGAAGCTATTGTAACTAC |
| XylFII-N151A-F | GAAAAACGTAAAGGCTCAGAAAGAAAGG |
| XylFII-N151A-R | CCTTTCTTTCTGAGCCTTTACGTTTTTC |
| XylFII-R155A-F | GAATCAGAAAGAAGCGGTTGAAGGCTTTAC |
| XylFII-R155A-R | GTAAAGCCTTCAACCGCTTCTTTCTGATTC |
| XylFII-D231A-F | AAATTATATGCTTTGCTGATTTGGATGATAC |
| XylFII-D231A-R | GTATCATCCAAATCAGCAAAGCATATAATTT |
| XylFII-Q251A-F | GCAACAATAGTGGCGAAAAGTAATGAA |
| XylFII-Q251A-R | TTCATTACTTTTCGCCACTATTGTTGC |
| XylFII-A70W-F | GATATGGCTACATCATGGAAGGTGAGTGGC |
| XylFII-A70W-R | GCCACTCACCTTCCATGATGTAGCCATATC |
| XylFII-D65A/M66G-F | GGGCTAAAACTTTTTGCTGGGGCTACATCAGCAAAG |
| XylFII-D65A/M66G-R | CTTTGCTGATGTAGCCCCAGCAAAAAGTTTTAGCCC |
| XylFII-M6A-F | GATTCATCGGATGCGGCGGCTGCTGCGGCAGCAGCCATAACAAGAAAAATATTAAATAG |
| XylFII-M6A-R | CTATTTAATATTTTTCTTGTTATGGCTGCTGCCGCAGCAGCCGCCGCATCCGATGAATC |
| LytS-M4-F | CTGATGATGTAAATGCAGCTATTTTAGCAGCTCTAGATAATACTTTAG |
| LytS-M4-R | CTAAAGTATTATCTAGAGCTGCTAAAATAGCTGCATTTACATCATCAG |
| LytS-M3-F | TTAAGAGATCTAGATGCTGCTTTAGCTTCTTATATAGAAAG |
| LytS-M3-R | CTTTCTATATAAGAAGCTAAAGCAGCATCTAGATCTCTTAA |
| XylFII-T53A/T54A-F | GAATTTTTAGGACCTGCAGCTGCAAGCACTGAAG |
| XylFII-T53A/T54A-R | CTTCAGTGCTTGCAGCTGCAGGTCCTAAAAATTC |
| XylFII-M6B-F | GATTCATCGGATGCGGAGGCTGAGGCGGCAGCAGCC |
| XylFII-M6B-R | GGCTGCTGCCGCCTCAGCCTCCGCATCCGATGAATC |