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. 2017 Jul 17;114(31):E6410–E6419. doi: 10.1073/pnas.1620598114

Fig. S3.

Fig. S3.

NaPy augments the KCNQ4 current by raising intracellular zinc. (A) Perforated patch-clamp recordings from KCNQ4-transfected CHO cells showing the time courses for the effects of 10 μM NaPy, 10 μM ZnPy, and 20 μM TPEN (as labeled) on the amplitude of the KCNQ4 current. Examples of current traces are shown on the right. The voltage protocol is given in the Inset above the traces. (B) Summary of the experiments presented in A. The number of cells is indicated within the bars; asterisks indicate significant difference from the baseline; **P < 0.01 (paired t test). (C) Fluorescence imaging of CHO cells loaded with the zinc fluorophore FluoZin-3 AM. Mean time course of changes in FluoZin-3 fluorescence during the application of 10 μM NaPy followed by 10 μM ZnPy; n = 31.