Fig. S8.

ZnPy reduces the inhibition of Kv7.4 by M1 muscarinic receptor activation. (A) Perforated patch-clamp recording from KCNQ4-transfected CHO cells showing the time courses for the effects of 20 μM Oxo-M and 10 μM XE991 (as labeled) on the amplitude of the KCNQ4 current. (B) As in A, but 10 μM ZnPy was applied before Oxo-M. (C) Summary of the experiments presented in A and B. The number of cells is indicated within the bars. Asterisks and number symbols denote a significant difference between groups indicated by the line connector; *P < 0.05, ***P < 0.001, and ^###P < 0.001 (paired or unpaired t test, as appropriate).