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. 2017 Jul 17;114(31):8229–8234. doi: 10.1073/pnas.1702192114

Fig. S7.

Fig. S7.

Modulation effect of TET3 on TRα1 is independent of DNA binding abilities of both TRα1 and TET3. (A) Effect of TET3/TET3ΔCXXC on the subcellular distribution of TRα1. HEK293T cells were cotransfected with indicated plasmids. After cell fractionation cytosol, nucleus and chromatin fractions were subjected to Western blotting. TET3/TET3ΔCXXC or TRα1 were, respectively, detected by anti-Flag and anti-GS antibody. β-tubulin, actin, and H3 were, respectively, the loading controls for the cytosol, nucleus, and chromatin. (B) Effect of TET3 on the subcellular distribution of TRα1G75S. Same cell fractionations were performed as in (A) for cells transfected with indicated plasmids. TET3 and TRα1G75S were respectively detected by anti-Flag and anti-GS antibody. (C) Interaction between TET3 and TRα1G75S. Whole-cell extracts from HEK293T cells cotransfected with indicated plasmids, treated or not with T3 (5.10–8 M) before collection, were used for Immunoprecipitation using M280 beads that retain GS tag. Coprecipitated TET3-Cat or TET3 were detected using an anti-Flag antibody.