Fig. 2.

Comprehensive quantification of fitness effects. Background-averaged fitness effects for 116 evolved mutations are quantified by the bulk-segregant fitness assay. Mutations are separated by lineage of origin and ordered by mean fitness effect. Fitness effect is represented as the SE of regression (thick lines) and 95% confidence interval (thin lines). Mutations are considered neutral (blue) if the confidence interval encompasses zero. Beneficial (orange) and deleterious (purple) mutations possess confidence intervals that fall completely above or below zero, respectively.