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Reiterative transcripts in the RTC crystals. In crystallo transcription on pyrG promoter scaffold (Fig. 2A) was performed in the presence of 400 µM GTP and [γ-32P]GTP. (Lane 1) RNAs released from crystals to supernatant in the course of reaction. (Lanes 2 and 3) RNAs released from crystals during two consecutive washes with the crystallization solution without GTP. (Lane 4) RNAs retained in the RTC crystals.
