Fig. S5.

Reiterative transcription with the synthetic scaffold and rifampin-resistant RNAPs in vitro. Transcription assays were performed in the presence of GTP {100 μM GTP plus 1 μCi [γ-³²P]GTP} and different concentrations of CTP (0, 10, 100, 500, or 1,000 μM). E. coli RNAPs including the wild-type and rifampin-resistant enzymes used for the reactions are indicated. The positions of the reiterative poly-G and the poly-G + C products are indicated. Note that poly-G transcripts migrate slower than same-length poly-G + C transcripts.