Fig. 2.

MIC calculation with unlabeled FPTMP and in vitro uptake of [¹⁸F]FPTMP with different species of bacteria. (A) A MIC experiment was performed by varying concentrations of TMP and FPTMP with S. aureus, E. coli, and P. aeruginosa. Incubation occurred overnight at 37 °C, and luminescence (relative luminescence units, RLU) assessment was performed the next day. (B and C) Time course of uptake and competition in the bacterial cultures at 37 °C in terms of percentage of injected dose (%ID) per colony forming units (cfu × 10⁸). Error bars represent the SD (n = 3). (D) Fold change of uptake in cultures of S. aureus, E. coli, and P. aeruginosa after a 3-h incubation with and without excess unlabeled (cold) TMP (10 μM). SA, S. aureus; EC, E. coli; PA, P. aeruginosa.