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Purity of His-tagged recombinant NRX1 proteins. E. coli expressing WT and cysteine mutants of His6-NRX1 were treated with IPTG and total protein extracted. His6-NRX1 proteins were purified by incubation with His-binding cobalt resin, washed, and eluted with imidazole. Proteins were separated by SDS/PAGE and stained with Coomassie blue.
