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. 2017 Jul 19;114(31):8414–8419. doi: 10.1073/pnas.1703344114

Fig. S2.

Fig. S2.

Identification and analysis of potential NRX1 substrates under stringent conditions. (A) Venn diagram illustrating proteins identified in the substrate capture experiment with an additional requirement that the number of peptides detected and used in quantification was equal to or more than two for each protein. Each circle represents proteins found to be enriched (P < 0.05, ratio > 1.5) in one of the following comparisons between columns: NRX1(C58,378S) column compared with WT NRX1 control column (pink); NRX1(C58,378S) column compared with resin-only control column (yellow); WT NRX1 control column compared with resin-only control column (blue). (B and C) GO term analysis was performed on the 45 targets (peptide count ≥ 2, see A) enriched in the immobilized NRX1(C58,378S) mutant column for biological process (B) and molecular function (C) using Classification Superviewer on bar.utoronto.ca/. Normalized frequencies, SD, and P values were determined from absolute values as described in ref. 36. Error bars represent SD. P values ≤ 0.05 are printed bold.