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. 2017 Jul 18;114(31):8283–8288. doi: 10.1073/pnas.1703718114

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Fig. 4. — Mutational study on CIB1 interacting with type III CD38. HEK293T cells (A) or the cells stably expressing Flag-tagged sCD38 (B) or Flag-tagged mutCD38 (C) were transfected with empty vector, HA-tagged CIB1 (CIB1-HA), G2A (G2A-HA), or S78A mutant (S78A-HA). CD38 in the lysates was immunoprecipitated, followed by Western blots with anti-Flag or anti-HA. The label (*) in C indicates type II CD38. Results from one representative experiment are shown. (D) Quantification of CIB1-HA, G2A-HA, or S78A-HA in the gels after IP shown in B and C. Values are represented as the mean ± SEM (n = 4). Additional results are shown in Fig. S6.