Fig. S8.

Mutational study on CIB1 interacting with type III CD38 using the BiFC assay. (A) Diagram of VC155-tagged wild-type CIB1 (CIB1-VC155) and CIB1 mutants (G2A-VC155 with the mutation of Gly to Ala at the second amino acid of CIB1 and ΔN5-VC155 with the deletion of first five amino acids of CIB1). (B) Expression of CIB1-VC155, G2A-VC155, ΔN5-VC155, or mutCD38-VN173 in HEK293T cells analyzed by Western blot with anti-CD38 or anti-HA antibody. Tubulin was used as a loading control. (C) Ratio of cells with BiFC signal in the cells coexpressing both mutCD38 and CIB1. Values were obtained by counting 280–344 cells (mean ± SEM).