Table S2.

Sequence analysis of FLiP genome and ORFs

ORF or gene	Direction	Nucleotide coordinates, start–stop	GC, %	Length, aa	Calculated. mass, kDa	Calculated isoelectric point	Protein	Function	Transmembrane helix predictions, n	Blast (E-value)	HHpred (% probable)
1	F	155–445	31.96	97	11.2	9.70
2	F	540–782	32.51	81	9.6	4.33
3	F	836–1,138	33.66	101	11.8	6.22			1
4	F	1,138–1,557	34.52	140	15.8	4.57					Helix-turn-helix motif (98.6)
5	F	1,799–2,008	39.52	70	7.1	10.5
6	F	2,011–2,229	31.05	73	9.0	9.80
7	F	2,703–2,957	33.73	85	10.3	12.92	gp7	S	1
8	F	3,036–3,971	37.07	312	34.6	5.07	gp8	S, MCP		Cellulophaga phage φ48:2 (0.020)
9	F	3,992–4,363	43.01	124	12.9	10.77	gp9	S	1	Cellulophaga phage φ12a:1 (5e-04)
10	F	4,385–4,861	35.01	159	18.5	6.83			1
11	F	4,845–5,321	37.32	159	17.9	9.42	gp11	S	1
12	F	5,324–5,770	33.56	149	17.0	4.63
13	F	5,770–6,780	35.61	337	38.4	4.31
14	F	6,780–7,445	37.39	222	25.2	9.61	gp14	S, lytic enzyme	1	Lytic enzyme (4e-28)	Lytic enzyme (99.8)
15	F	7,510–8,853	31.99	448	53.5	9.06				Cellulophaga phage φ48:2 (3e-12)	Replication initiation protein (97.0)
16	F	8,865–9,110	28.86	82	9.3	8.46					Coiled-coiled protein (92.1)

ORF or gene as in Fig. 1; nucleotide coordinates refer to FLiP genome; gene functions as predicted by BlastP, Psi-Blast, and HHpred programs. The E-value of the most relevant Blast match is indicated. The most relevant HHpred predictions are listed. F, forward; gp, confirmed by proteomics (see Table S3); R, reverse; S, structural protein.