Table S4.
Parameter | Value |
Cryo-EM data collection | |
Voltage, kV | 300 |
Magnification, × | 37,037 |
Defocus, μm | 0.7–2.5 |
Dose rate, electrons per pixels per second | 8 |
Frames | 22 |
Frame length, s | 0.2 |
Total dose, e−/Å2 | 22 |
Micrographs | 235 |
Cryo-EM data processing | |
Particles | 934 |
Box size, pixels | 700 |
Pixel size, Å | 1.35 |
Symmetry | I1 |
Resolution, Å | 4.0 |
Map sharpening B-factor, Å2 | −100 |