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. 2017 Jul 17;114(31):8378–8383. doi: 10.1073/pnas.1703834114

Table S4.

FLiP data collection and processing

Parameter Value
Cryo-EM data collection
 Voltage, kV 300
 Magnification, × 37,037
 Defocus, μm 0.7–2.5
 Dose rate, electrons per pixels per second 8
 Frames 22
 Frame length, s 0.2
 Total dose, e2 22
 Micrographs 235
Cryo-EM data processing
 Particles 934
 Box size, pixels 700
 Pixel size, Å 1.35
 Symmetry I1
 Resolution, Å 4.0
 Map sharpening B-factor, Å2 −100