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. 2017 Jul 17;114(31):E6427–E6436. doi: 10.1073/pnas.1704945114

Fig. 6.

Fig. 6.

HDAC4 mRNA and protein levels in human cervical stromal tissues at different stages of pregnancy. (A) HDAC4 and 15-PGDH mRNA levels quantified in human cervical stromal tissues obtained from nonpregnant (NP, n = 7), pregnant early gestation (E. gest, n = 4), pregnant with cervix not ripe and not in labor (near term, Not Ripe, n = 7), term pregnant with cervix ripe before labor (Ripe, n = 8), and term pregnant in labor (IL, n = 7). Data represent relative mRNA levels normalized to GAPDH mRNA levels. *P < 0.05, **P < 0.001 compared to nonpregnant. NS, not significant. ANOVA followed by Tukey’s multiple comparisons test. AU, arbitrary unit. (B) Immunolocalization of HDAC4 in human cervical stroma of (a) nonpregnant (NP) and (b) pregnant women in early gestation (10–14 wk), at term before cervical ripening (Not Ripe), (c) term after cervical ripening but before labor (Ripe), and (d) in labor (In Labor). Results were consistent, representing 3–4 tissues in each group. (Scale bar, 20 µm.)