Binding of plasma IgG derived from ZIKV- and DENV-immune donors to NS1 antigens from different flaviviruses. (A–C) Plasma from ZIKV-immune (A), DENV-immune (B), or heathy (C) donors were tested at 1:100 dilution for binding of total IgG to wells coated with ZIKV, DENV1–DENV4, WNV, or YFV NS1. Samples from ZIKV-immune donors known to be DENV-naive, DENV-immune, or unknown are shown in gray, black, and white symbols, respectively. Samples from DENV-immune donors with secondary DENV infections are shown in dark gray.