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. 2017 Jul 17;114(31):E6371–E6380. doi: 10.1073/pnas.1706504114

Fig. S8.

Fig. S8.

Vps34f/f and Vps34f/f;CD11c-Cre DC cocultures. (A) Wild-type (CD45.1) DCs and Vps34f/f or Vps34f/f;CD11c-Cre (CD45.2) DCs were cultured alone or were cocultured at a ratio of 1:1 at a final density of 4 × 105 cells per well in U-bottomed plates. After 24 h, the expression of TIM-4 was measured on the surface of CD45.2+CD8α+ DCs. (B) Wild-type (CD45.1) splenocytes and Vps34f/f or Vps34f/f;CD11c-Cre (CD45.2) DCs were cultured alone or were cocultured at a 1:1 ratio at a final density of 4 × 105 cells per well in U-bottomed plates. After 24 h, the expression of TIM-4 was measured on the surface of CD45.2+CD8α+ DCs. (C) Vps34f/f;CD11c-Cre (CD45.2) splenocytes were cocultured with wild-type (CD45.1) DCs or Vps34f/f;CD11c-Cre (CD45.2) DCs at a 1:1 ratio and at a final density of 4 × 105 cells per well in U-bottomed plates. After 24 h, the expression of TIM-4 was measured on the surface of CD8α+ DCs. An experiment representative of two independent experiments is shown. (D) TIM-4 expression was measured on the surface of CD8α+ DCs derived from Vps34f/f or Vps34f/f;CD11c-Cre mice stimulated with recombinant mouse IL-10 (10 ng/mL) for 24 h. An experiment representative of two independent experiments is shown.