Fig. 4.

Spz-3 and Toll-8 signaling pathway is involved in melanin pigmentation in Ze and p^S larvae. (A) Color pattern of the Ze mutant (Left) and another striped mutant p^S (Right). The p^S mutant has a broader black stripe in each segment than Ze. (Scale bars: 1 cm.) (B) Phenotypic changes of larva and cuticle introduced by siRNA of Bmspz-3 and relative expression levels of Bmspz-3 between the introduced (+) and nonintroduced (‒) sides of the p^S mutant. (Scale bars: 1 mm.) (C and D) Phenotypic changes of larva and cuticle introduced by siRNA of BmToll-8 and relative expression levels of BmToll-8 between the introduced (+) and nonintroduced (‒) sides of the Ze (C) and p^S (D) mutants. Error bars indicate the SD. Statistical comparisons were conducted using the one-sided paired Student’s t test. (Scale bars: 1 mm.)