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Phylogenetic analysis and knockdown of spz family genes other than spz-3 in the Ze and pS strains. (A) The phylogenetic tree was inferred based on the amino acid sequences of the CK domain from public databases using the maximum likelihood method (LG + G + I model). Bootstrap values >50% are provided in the branches. (B) Phenotypes of knockdown of other spz family genes in Ze and pS strains. Dashed lines illustrate the position of the dorsal midline. (Scale bars: 1 mm.)
